Human Exosome Isolation
Introduction
I have sent many Freedom & Official Information Act Requests for the isolation of SARS-COV-2. Not a single organisation responded with any records. Critics could say that the question is invalid and that it's impossible to isolate anything as described. So I decided to challenge my critics by sending a new request; maintaining the exact same language but replacing SARS-COV-2 with human exosome. This document details the response that I have received.
Exosomes are relevant because exosomes are nearly identical to virus physically. This means the isolation techniques used for exosomes are directly applicable to virus. Here is a chart that lists the similarities of exosomes and virus.
Virus | Exosome |
|---|---|
30nm to 160nm | 30nm to 160nm |
Has Receptors | Has Receptors |
Single Stranded RNA | Single Stranded RNA |
Buds out of Cells | Buds out of Cells |
Manufactured inside the Cell | Manufactured inside the Cell |
Request Comparison
The table below shows the language used in both the requests for information. The only significant difference, is the use of the word SARS-COV-2 and exosome.
Isolation of SARS-COV-2 | Isolation of Human Exosomes |
|---|---|
University of Otago’s Response
The University of Otago has responded with a massive 331 documents relating to the isolation of human exosomes. They wrote the following:
I had previously identified a few scientific papers that isolate human exosomes from unadulterated samples. One of the papers that I had identified was included in the Universities Response. The paper that I have personally verified is below:
14. Baranyai T, Herczeg K, Onódi Z, et al. Isolation of exosomes from blood plasma: Qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods. PLoS One, 2015 - link
The method and materials section is very detailed and describes the process of using Ultra Filtration, Ultra Centrifugation, Size-exclusion Chromatography, and Precipitation to actually isolate the exosomes. After the exosomes were isolated by the methods listed above, the exosomes were visualised by the Transmission Electron Microscopy (TEM).
This methods and materials section is a scientific process that correctly identifies and proves the existence of exosomes! This process has NOT been followed for the isolation of SARS-COV-2 !
Here is the official response from University of Otago.
References
I will be working through the 331 references in the Official Response. As indicated by the response, at least some of the responses isolate exosomes from unadulterated samples. I am curious to see how many of the 331 meet the definition as I have described.
This section lists the references received in the Official Information Act Responses. WORK IN PROGRESS.